Marker-assisted identification of maize genotypes with improved protein quality

Production of Marker-free Transgenic Rice (Oryza sativa L.) with Improved Nutritive Quality Expressing AmA1

Background: Rice seed proteins are lacking essential amino acids (EAAs). Genetic engineering off ers a fast and sustainable method to solve this problem as it allows the specifi c expression of heterologous EAA-rich proteins. The use of selectable marker gene is essential for generation of transgenic crops, but might also lead to potential environmental and food safety problems...

Identification of Gene Markers in Aflatoxin-Resistant Maize Germplasm for Marker-Assisted Breeding

Marker assisted characterization of wilt resistance in productive Chickpea genotypes

Fusarium wilt caused by Fusarium oxysporum f. sp. ciceris is one of the most wide spread disease of chickpea (Cicer arietinum). Studies indicated two major independent loci H1 and H2 determine the resistance to race-1 in chickpea. The dominant alleles at both H1 and H2 loci result in susceptible early wilting and recessive at any one (h1h1H2_ or H1_h2h2) produce susceptible late wilting and rec...

Marker-Assisted Selection for Recognizing Wheat Mutant Genotypes Carrying HMW Glutenin Alleles Related to Baking Quality

Allelic diversity of HMW glutenin loci in several studies revealed that allelic combinations affect dough quality. Dx5 + Dy10 subunits are related to good baking quality and Dx2 + Dy12 are related to undesirable baking quality. One of the most regular methods to evaluate the baking quality is SDS-PAGE which is used to improve baking quality labs. Marker-assisted selection is the method which ca...

Molecular marker-assisted selection of the ae alleles in maize.

The ae (amylose extender) recessive mutant alleles in maize are an important genetic resource for the development of high-amylose cultivars. On the basis of ae allele sequences (from the National Center for Biotechnology Information), the ae mutant alleles were cloned from high-amylose maize and the allelic Ae gene from common maize luyuan92 inbred lines. Five pairs of primers were designed to ...